Gene description for LTF |
Gene name |
lactotransferrin |
Gene symbol |
LTF |
Other names/aliases |
GIG12 HEL110 HLF2 LF |
Species |
Homo sapiens |
Experiment description of studies that identified LTF in Breast milk |
1
|
Experiment ID | 46 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
17641064
|
Organism | Homo sapiens |
Experiment description | Exosomes with immune modulatory features are present in human breast milk. |
Authors | Admyre C, Johansson SM, Qazi KR, FiléJJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S |
Journal name |
JIMMU
|
Publication year | 2007 |
Sample | Breast milk |
Sample name | Breast milk - colostrum |
Isolation/purification methods | Differential centrifugation Filtration Sucrose density gradient |
Flotation density | 1.10-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [QSTAR] Western blotting Immunoelectron microscopy FACS |
EV-TRACK |
-
|
|
|
2
|
Experiment ID | 48 |
Identified molecule | protein
|
Extracellular vesicle type | Exosomes |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
17641064
|
Organism | Homo sapiens |
Experiment description | Exosomes with immune modulatory features are present in human breast milk. |
Authors | Admyre C, Johansson SM, Qazi KR, FiléJJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S |
Journal name |
JIMMU
|
Publication year | 2007 |
Sample | Breast milk |
Sample name | Breast milk - mature milk |
Isolation/purification methods | Differential centrifugation Filtration Sucrose density gradient |
Flotation density | 1.10-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [QSTAR] Western blotting Immunoelectron microscopy FACS |
EV-TRACK |
-
|
|
|
3
|
Experiment ID | 574 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ-Orbitrap Elite, Q-Exactive]
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components |
Authors | Van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
MCP
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Breast milk - normal |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
4
|
Experiment ID | 950 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - D1 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
5
|
Experiment ID | 951 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - D2 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
6
|
Experiment ID | 952 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - D3 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
7
|
Experiment ID | 953 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - D4 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
8
|
Experiment ID | 954 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - D5 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
9
|
Experiment ID | 955 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - D6 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
10
|
Experiment ID | 956 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - D7 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
11
|
Experiment ID | 957 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
27601599
|
Organism | Homo sapiens |
Experiment description | Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. |
Authors | van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH |
Journal name |
Mol Cell Proteomics
|
Publication year | 2016 |
Sample | Breast milk |
Sample name | Milk - derived EVs - pooled |
Isolation/purification methods | Differential centrifugation Ultracentrifugation Density gradient centrifugation |
Flotation density | 1.12-1.18 g/mL |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
EV160000: EV-METRIC:50%
|
|
|
12
|
Experiment ID | 2198 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 1 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
13
|
Experiment ID | 2199 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 2 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
14
|
Experiment ID | 2200 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 3 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
15
|
Experiment ID | 2201 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 4 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
16
|
Experiment ID | 2202 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 5 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
17
|
Experiment ID | 2203 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 6 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
18
|
Experiment ID | 2204 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 7 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
19
|
Experiment ID | 2205 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 8 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|
20
|
Experiment ID | 2206 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Western blotting
|
PubMed ID |
33732416
|
Organism | Homo sapiens |
Experiment description | Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses |
Authors | Zonneveld MI, van Herwijnen MJC, Fernandez-Gutierrez MM, Giovanazzi A, de Groot AM, Kleinjan M, van Capel TMM, Sijts AJAM, Taams LS, Garssen J, de Jong EC, Kleerebezem M, Nolte-'t Hoen ENM, Redegeld FA, Wauben MHM. |
Journal name |
J Extracell Vesicles
|
Publication year | 2021 |
Sample | Breast milk |
Sample name | Eluate 9 |
Isolation/purification methods | Differential centrifugation OptiPrep density gradient centrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting |
EV-TRACK |
-
|
|
|